Initially it seems Apache is not configured to serve index.php as a top-level file. @brady.miller has there been any recent changes to the XAMPP package that may have caused this?
@Ashlee_McPherson can you open the XAMPP Control Panel program
Next, search for “DirectoryIndex” You should find a section of the text file that looks similar to this:
If the following text is not present on that line, please add it:
Save the file, restart Apache from the XAMPP Control Panel
Navigate to http://localhost/openemr have the changes solved the problem?